Finlands Akademi
Namn Groenhof, Gerrit
Organisation JY
Projektets titel FluProCad: a software package for computer aided design of fluorescent proteins
Beslutnr 304455
Beslutsdatum 30.09.2016
Finansieringsperiod 01.10.2016 - 30.09.2018
Finansiering 268 360
WebFOCUS Report
Beskrivning av projektet
Fluorescence microscopy with fluorescent proteins is an essential technique for revealing how cells function and for developing new strategies to fight diseases. After fusing a fluorescent protein to another protein using gene technology, the exact location of that protein can be determined by monitoring the fluorescence emitted by the photo-excited fluorescent protein with a normal microscope. The resolution of this technique is determined by the properties of the fluorescent proteins, rather than lenses. However, these proteins still require significant improvements of their properties to unlock the full potential of this technique for biological imaging. To overcome this limitation, we develop a software package that performs atomistic simulations based on a validated physical model. From the simulations, all relevant properties of the protein are extracted, such as protein stability, absorption and fluorescence spectra, as well as quantum yields.